In standard Mascot Server peptide summary reports, there is an "unassigned list" at the bottom of the page - for example:
Unassigned queries: (no details means no match)
Query Observed Mr(expt) Mr(calc) Delta Miss Score Rank Peptide
113 893.28 1784.54 1784.92 -0.37 0 65 1 VGAVAGNDWLIWDITR
107 882.21 1762.40 1762.79 -0.38 0 62 1 LISWYDNEFGYSNR
67 691.20 1380.39 1380.64 -0.24 0 61 1 ALEESNYELEGK
70 695.73 1389.45 1389.67 -0.21 0 60 1 QSLEASLAETEGR
This is a list of MS-MS spectra that have had matches to peptides, but are not included in the main report because the proteins which they are found in have too low a score. If the list of proteins in the main report was not cut off, i.e. if all proteins were displayed, then there would be no unassigned list.
For example, if a query had the following matches, then it would not be in the unassigned list.
Although there was no protein match the rank 1 peptide, the rank 2 peptide had the same score, so this is in effect treated as the top match for the query. The query will then be included under
An unassigned list can be created for a protein summary. This list is just the list of peptides that didn't get a match anywhere in the top 50 hits.
Sort the unassigned list by ascending query number. This is the same as ascending relative mass order.
Sort the unassigned list by descending score.
Sort the unassigned list by descending intensity. Intensity values are taken from the
qintensity value in the results file if they are available (pkl files, some MGF files). If these values are not available, then the intensity is calculated from the sum of all the ions values.
Note that for a very large MS-MS file, this option can take some time to process unless there are
qintensity values in the results file.
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