Example program for extracting the peptide or protein summary.
/* ############################################################################## # file: resfile_summary.cpp # # 'msparser' toolkit example code # ############################################################################## # COPYRIGHT NOTICE # # Copyright 1998-2005 Matrix Science Limited All Rights Reserved. # # # ############################################################################## # $Source: parser/examples/test_cxx/resfile_summary.cpp $ # # $Author: villek@matrixscience.com $ # # $Date: 2018-07-30 16:23:53 +0100 $ # # $Revision: 1b450440f9c97e1e41d0fc6016a27d68951d4532 | MSPARSER_REL_2_8_1-0-gea32989045 $ # # $NoKeywords:: $ # ############################################################################## */ #include "msparser.hpp" #include <iostream> #include <iomanip> #include <fstream> // All the classes are part of the matrix_science namespace using namespace matrix_science; /*****************************************************************************/ /* Local functions */ /*****************************************************************************/ static void displayPeptideInfo(bool showFullDetails, ms_peptide * pep, ms_mascotresults * r, bool isDuplicate, bool isBold, bool showCheckBox); static bool showResults(ms_mascotresfile & file, bool peptideSummary, const int flags, const double minProbability = 0, const int maxHitsToDisplay = 50, const double ignoreIonsScoreBelow = 0, const int minPepLenInPepSummary = 0, const int flags2 = ms_peptidesummary::MSPEPSUM_NONE); static void displayYellowPopupInfo(ms_mascotresults * results, int q); static bool checkErrors(ms_mascotresfile & file); /*****************************************************************************/ /* main */ /* Call this program with a single argument - the name of the .dat file */ /*****************************************************************************/ int main(int argc, char * argv[]) { int returnValue = EXIT_FAILURE; if (argc == 2) { ms_mascotresfile file(argv[1]); if (checkErrors(file)) { bool usePeptideSummary; unsigned int flags, flags2, minPepLenInPepSummary; int maxHitsToReport; double minProbability, ignoreIonsScoreBelow; /* * The parameters passed to ms_peptidesummary or ms_proteinsummary determine * the type of grouping and the number of proteins and peptides displayed. * Default parameters can be returned using ms_mascotresfile::get_ms_mascotresults_params(). * The return values from this function depend on the type of search, * and also on values in the mascot.dat configuration file if that is available. */ ms_datfile datfile("../config/mascot.dat"); //You may need to change this path //if the mascot.dat isn't available, use defaults ms_mascotoptions mascotOptions; if (datfile.isValid()) { mascotOptions = *(datfile.getMascotOptions()); } std::string scriptName = file.get_ms_mascotresults_params( mascotOptions, &flags, &minProbability, &maxHitsToReport, &ignoreIonsScoreBelow, &minPepLenInPepSummary, &usePeptideSummary, &flags2); bool bResult; if (usePeptideSummary) { /* * For peptide summary * * Flags defined for hierarchical clustering algorithm: * flags: ms_mascotresults::MSRES_CLUSTER_PROTEINS * | ms_mascotresults::MSRES_SHOW_SUBSETS * | ms_mascotresults::MSRES_MUDPIT_PROTEIN_SCORE; * flags2: ms_peptidesummary::MSPEPSUM_USE_HOMOLOGY_THRESH; * * Flags defined for original simple parsimony algorithm: * flags: ms_mascotresults::MSRES_GROUP_PROTEINS | ms_mascotresults::MSRES_SHOW_SUBSETS; */ std::cout << "-------------------------------------------------------------" << std::endl; std::cout << "--- Peptide summary report ---" << std::endl; std::cout << "-------------------------------------------------------------" << std::endl; bResult = showResults(file, usePeptideSummary, flags, minProbability, maxHitsToReport, ignoreIonsScoreBelow, minPepLenInPepSummary, flags2); } else { // Show results from full protein summary, remove grouping flags &= ~ms_mascotresults::MSRES_GROUP_PROTEINS; flags &= ~ms_mascotresults::MSRES_SHOW_SUBSETS; std::cout << "-------------------------------------------------------------" << std::endl; std::cout << "--- Full Protein summary report ---" << std::endl; std::cout << "-------------------------------------------------------------" << std::endl; bResult = showResults(file, usePeptideSummary, flags, minProbability, maxHitsToReport, ignoreIonsScoreBelow, minPepLenInPepSummary, flags2); if (bResult && checkErrors(file)) { std::cout << std::endl; // Show results from concise protein summary, add grouping flags |= ms_mascotresults::MSRES_GROUP_PROTEINS; flags |= ms_mascotresults::MSRES_SHOW_SUBSETS; std::cout << "-------------------------------------------------------------" << std::endl; std::cout << "--- Concise Protein summary report ---" << std::endl; std::cout << "-------------------------------------------------------------" << std::endl; bResult = showResults(file, usePeptideSummary, flags, minProbability, maxHitsToReport, ignoreIonsScoreBelow, minPepLenInPepSummary, flags2); } } if (bResult && checkErrors(file)) { returnValue = EXIT_SUCCESS; } } } else { std::cout << "Must supply the name of a .dat file as a command line argument" << std::endl; } return returnValue; } /*****************************************************************************/ /* showResults */ /* Shows results using either the peptide summary or the protein summary */ /*****************************************************************************/ static bool showResults(ms_mascotresfile & file, bool peptideSummary, const int flags, const double minProbability, const int maxHitsToDisplay, const double ignoreIonsScoreBelow, const int minPepLenInPepSummary, const int flags2) { ms_mascotresults * results; if (peptideSummary) { results = new ms_peptidesummary(file, flags, minProbability, maxHitsToDisplay, 0, ignoreIonsScoreBelow, minPepLenInPepSummary, "", flags2); } else { results = new ms_proteinsummary(file, flags, minProbability, maxHitsToDisplay); } //Stop there is any fatal error occurred if (!checkErrors(file)) { return false; } int family = 1; int hit = 1; ms_protein * prot = results->getHit(hit); while (prot) { std::string accession = prot->getAccession(); std::string description = results->getProteinDescription(accession.c_str()); double mass = results->getProteinMass(accession.c_str()); int dbIdx = prot->getDB(); std::cout << "Protein Hit " << hit; if (flags & ms_mascotresults::MSRES_CLUSTER_PROTEINS) { std::cout << "." << family; } std::cout << "\n===================\n"; std::cout << "Accession : " << accession << std::endl; std::cout << "Description : " << description << std::endl; std::cout << "Score : " << prot->getScore() << std::endl; std::cout << "Mass : " << mass << std::endl; std::cout << "Frame : " << prot->getFrame() << std::endl; std::cout << "Coverage : " << prot->getCoverage() << std::endl; std::cout << "Peptides : " << prot->getNumDisplayPeptides()<< std::endl; int i; // Each protein has a number of peptides that matched - list them: for (i=1; i <= prot->getNumPeptides(); i++) { int query = prot->getPeptideQuery(i); int p = prot->getPeptideP(i); if (p != -1 && query != -1 && prot->getPeptideDuplicate(i) != ms_protein::DUPE_DuplicateSameQuery) { ms_peptide * pep; if (results->getPeptide(query, p, pep)) { displayPeptideInfo(false, pep, results, prot->getPeptideDuplicate(i) == ms_protein::DUPE_Duplicate, prot->getPeptideIsBold(i), prot->getPeptideShowCheckbox(i)); } } } // Now display list of all proteins that contain a subset or a same set of the matching peptides if ( (flags & ms_mascotresults::MSRES_GROUP_PROTEINS) || (flags & ms_mascotresults::MSRES_CLUSTER_PROTEINS) ) { std::cout << "Proteins matching the same set of peptides:" << std::endl; i = 1; while ((prot = results->getNextSimilarProteinOf(accession.c_str(), dbIdx, i)) != 0) { std::string accession = prot->getAccession(); if (flags & ms_mascotresults::MSRES_CLUSTER_PROTEINS) { int dbIdx = prot->getDB(); if (dbIdx > 1) { std::cout << dbIdx << "::"; } } std::cout << accession.c_str() << " - Total score: " << prot->getScore() << " - Peptides matched: " << prot->getNumDisplayPeptides() << std::endl; i++; } if (flags & ms_mascotresults::MSRES_SHOW_SUBSETS) { std::cout << "Proteins matching a subset of these peptides:" << std::endl; i = 1; while ((prot = results->getNextSubsetProteinOf(accession.c_str(), dbIdx, i)) != 0) { std::string accession = prot->getAccession(); int dbIdx = prot->getDB(); if ( (flags & ms_mascotresults::MSRES_CLUSTER_PROTEINS) && dbIdx > 1) { std::cout << dbIdx << "::"; } std::cout << accession.c_str() << " - Total score: " << prot->getScore() << " - Peptides matched: " << prot->getNumDisplayPeptides() << std::endl; if (flags & ms_mascotresults::MSRES_CLUSTER_PROTEINS) { int j = 1; if (results->getNextSimilarProteinOf(accession.c_str(), dbIdx, j)) { std::cout << " Proteins matching the same set of peptides for this subset:" << std::endl; } ms_protein * similar_subset_prot; while ((similar_subset_prot = results->getNextSimilarProteinOf(accession.c_str(), dbIdx, j)) != 0) { std::string similar_subset_accession = similar_subset_prot->getAccession(); int similar_subset_dbIdx = similar_subset_prot->getDB(); std::cout << " "; if (similar_subset_dbIdx > 1) { std::cout << similar_subset_dbIdx << "::"; } std::cout << similar_subset_accession.c_str() << " - Total score:" << similar_subset_prot->getScore() << " - Peptides matched: " << similar_subset_prot->getNumDisplayPeptides() << std::endl; j++; } } i++; } } } if (flags & ms_mascotresults::MSRES_CLUSTER_PROTEINS) { prot = results->getNextFamilyProtein(hit, family++); if (!prot) { hit++; prot = results->getHit(hit); family = 1; } } else { hit++; prot = results->getHit(hit); } std::cout << std::endl; } results->createUnassignedList(ms_mascotresults::SCORE); if (results->getNumberOfUnassigned()) { std::cout << "Unassigned list" << std::endl << "---------------" << std::endl; for (int u = 1; u <= results->getNumberOfUnassigned(); u++) { ms_peptide pep = results->getUnassigned(u); displayPeptideInfo(false, &pep, results, false, true, true); } } if (peptideSummary) displayYellowPopupInfo(results, 1); delete results; return true; } /*****************************************************************************/ /* displayYellowPopupInfo */ /* Shows the equivalent of the yellow popup box for given query */ /*****************************************************************************/ static void displayYellowPopupInfo(ms_mascotresults * results, int q) { std::cout << "Score Delta Hit Protein Peptide" << std::endl; int p; for (p=1; p <= 10; p++) { ms_peptide pep = results->getPeptide(q, p); std::string seq = pep.getPeptideStr(); if (!seq.empty()) { std::string tmp = results->getProteinsWithThisPepMatch(q, p, true); std::string hit = " "; std::string protein = " "; if (!tmp.empty()) { hit = tmp.substr(0, tmp.find(':')); std::string::size_type start = tmp.find(":\""); if (start != tmp.rfind(":\"")) hit += "+"; protein = tmp.substr(2 + start, tmp.find("\" ") - start -2); } std::cout << pep.getIonsScore() << " " << pep.getDelta() << " " << hit << " " << protein << " " << pep.getPeptideStr() << std::endl; } } p = 1; std::cout << "Accessions that matched query " << q << " rank " << p << ":- " << results->getProteinsWithThisPepMatch(q, p) << std::endl << std::endl; } /*****************************************************************************/ /* displayPeptideInfo */ /* Shows information about a peptide */ /*****************************************************************************/ static void displayPeptideInfo(bool showFullDetails, ms_peptide * p, ms_mascotresults * r, bool isDuplicate, bool isBold, bool showCheckBox) { int q = p->getQuery(); if (showFullDetails) { std::cout << "Peptide hit" << std::endl; if (p->getAnyMatch()) { std::cout << " Query : " << q << std::endl; std::cout << " Rank : " << p->getRank() << std::endl; std::cout << " Matched : " << p->getAnyMatch() << std::endl; std::cout << " missedCleave: " << p->getMissedCleavages() << std::endl; std::cout << " mrCalc : " << p->getMrCalc() << std::endl; std::cout << " delta : " << p->getDelta() << std::endl; std::cout << " observed : " << p->getObserved() << std::endl; std::cout << " charge : " << p->getCharge() << std::endl; std::cout << " mrExp : " << p->getMrExperimental() << std::endl; std::cout << " ionsMatched : " << p->getNumIonsMatched() << std::endl; std::cout << " peptideStr : " << p->getPeptideStr() << std::endl; std::cout << " peaksUsed1 : " << p->getPeaksUsedFromIons1() << std::endl; std::cout << " varModsStr : " << p->getVarModsStr() << std::endl; std::cout << " readable mod: " << r->getReadableVarMods(q, p->getRank()) << std::endl; std::cout << " tag matches : " << r->getTagString(q, 1) << std::endl; std::cout << " first tag : 1:" << r->getTagStart(q,1,1) << ":" << r->getTagEnd(q,1,1) << ":" << r->getTagSeries(q,1,1) << std::endl; std::cout << " etag drange : " << r->getTagDeltaRangeStart(q, 1) << "," << r->getTagDeltaRangeEnd(q, 1) << std::endl; std::cout << " ionsScore : " << p->getIonsScore() << std::endl; std::cout << " seriesUsed : " << p->getSeriesUsedStr() << std::endl; std::cout << " peaksUsed2 : " << p->getPeaksUsedFromIons2() << std::endl; std::cout << " peaksUsed3 : " << p->getPeaksUsedFromIons3() << std::endl; std::cout << " idth, hth, p: " << r->getPeptideIdentityThreshold(q,20) << ", " << r->getHomologyThreshold(q, 20) << ", " << r->getProbOfPepBeingRandomMatch(p->getIonsScore(), q) << std::endl; } else std::cout << " No match" << std::endl; } else { if (showCheckBox) std::cout << "CB "; else std::cout << "-- "; if (isBold) std::cout << "BOLD "; else std::cout << "dim "; std::cout << std::setiosflags(std::ios::left) << std::setw(4) << std::setfill(' ') << q; std::cout << std::setiosflags(std::ios::left) << std::setw(11) << std::setfill(' ') << p->getObserved(); std::cout << std::setiosflags(std::ios::left) << std::setw(3) << std::setfill(' ') << p->getRank(); std::cout << "("; std::cout << std::setiosflags(std::ios::left) << std::setw(3) << std::setfill(' ') << p->getPrettyRank(); std::cout << ")"; std::cout << std::setiosflags(std::ios::left) << std::setw(20) << std::setfill(' ') << p->getPeptideStr() << "\t"; if (isDuplicate) std::cout << "(" << p->getIonsScore() << ")"; else std::cout << p->getIonsScore(); std::cout << "\t"; std::cout << r->getReadableVarMods(q, p->getRank()); std::cout << std::endl; } } /*****************************************************************************/ /* checkErrors */ /* Print errors and return false if a critical error occurred */ /*****************************************************************************/ static bool checkErrors(ms_mascotresfile & file) { if (file.getLastError()) { for (int i=1; i <= file.getNumberOfErrors(); i++) { std::cout << "Error number: " << file.getErrorNumber(i) << " : " << file.getErrorString(i) << std::endl; } } //Call isValid before clearAllErrors, otherwise this method always returns true bool bIsValid = file.isValid(); file.clearAllErrors(); return bIsValid; } /* will give the output: ------------------------------------------------------------- --- Peptide summary report --- ------------------------------------------------------------- Protein Hit 1 =================== Accession : Q9XZJ2 Description : HEAT SHOCK PROTEIN 70.- Crassostrea gigas (Pacific oyster). Score : 286.477 Mass : 79480.1 Frame : 0 Coverage : 57 CB BOLD 1 671.9 1 (1 )DAGTISGLNVLR 95.12 CB BOLD 2 808.3 1 (1 )TTPSYVAFTDTER 74.55 CB BOLD 3 973.9 1 (1 )NQVAMNPNNTIFDAK 89.84 -- BOLD 4 1084.9 3 (3 )IINEPTAAAIAYGLDKK 30.07 Proteins matching the same set of peptides: Q94805 Total score: 283.374 Proteins matching a subset of these peptides: AAF09496 Total score: 211.927 Peptides matched: 3 BAB72169 Total score: 199.74 Peptides matched: 3 Q24789 Total score: 199.74 Peptides matched: 3 Q9I9Q5 Total score: 199.74 Peptides matched: 3 AAB93665 Total score: 196.637 Peptides matched: 3 Q9W6Y1 Total score: 196.637 Peptides matched: 3 Q95V47 Total score: 194.46 Peptides matched: 3 Q91993 Total score: 193.534 Peptides matched: 3 T45476 Total score: 191.357 Peptides matched: 3 O01948 Total score: 169.67 Peptides matched: 2 O97150 Total score: 166.567 Peptides matched: 2 AAA64872 Total score: 125.19 Peptides matched: 2 B44261 Total score: 125.19 Peptides matched: 2 P81159 Total score: 125.19 Peptides matched: 2 Q9NJ92 Total score: 125.19 Peptides matched: 2 Q9NJ93 Total score: 125.19 Peptides matched: 2 Q9PUF9 Total score: 125.19 Peptides matched: 2 S21175 Total score: 125.19 Peptides matched: 2 T45473 Total score: 125.19 Peptides matched: 2 JC4610 Total score: 122.087 Peptides matched: 2 O73788 Total score: 122.087 Peptides matched: 2 1ATR Total score: 104.62 Peptides matched: 2 1ATS Total score: 104.62 Peptides matched: 2 1BA0 Total score: 104.62 Peptides matched: 2 1BA1 Total score: 104.62 Peptides matched: 2 1BUPA Total score: 104.62 Peptides matched: 2 1HSC Total score: 104.62 Peptides matched: 2 1KAX Total score: 104.62 Peptides matched: 2 1KAY Total score: 104.62 Peptides matched: 2 1KAZ Total score: 104.62 Peptides matched: 2 1NGC Total score: 104.62 Peptides matched: 2 1NGD Total score: 104.62 Peptides matched: 2 1NGE Total score: 104.62 Peptides matched: 2 1NGF Total score: 104.62 Peptides matched: 2 1NGG Total score: 104.62 Peptides matched: 2 1NGH Total score: 104.62 Peptides matched: 2 1NGI Total score: 104.62 Peptides matched: 2 2BUPA Total score: 104.62 Peptides matched: 2 A25646 Total score: 104.62 Peptides matched: 2 A27077 Total score: 104.62 Peptides matched: 2 A35922 Total score: 104.62 Peptides matched: 2 A44985 Total score: 104.62 Peptides matched: 2 A45635 Total score: 104.62 Peptides matched: 2 A45935 Total score: 104.62 Peptides matched: 2 A48439 Total score: 104.62 Peptides matched: 2 AAA30130 Total score: 104.62 Peptides matched: 2 AAA30569 Total score: 104.62 Peptides matched: 2 AAA33009 Total score: 104.62 Peptides matched: 2 CAA81523 Total score: 104.62 Peptides matched: 2 HHKW7A Total score: 104.62 Peptides matched: 2 HHUM7B Total score: 104.62 Peptides matched: 2 JC2215 Total score: 104.62 Peptides matched: 2 JC4786 Total score: 104.62 Peptides matched: 2 JC5642 Total score: 104.62 Peptides matched: 2 O61003 Total score: 104.62 Peptides matched: 2 O61004 Total score: 104.62 Peptides matched: 2 O73885 Total score: 104.62 Peptides matched: 2 O76274 Total score: 104.62 Peptides matched: 2 O96541 Total score: 104.62 Peptides matched: 2 O96911 Total score: 104.62 Peptides matched: 2 P79984 Total score: 104.62 Peptides matched: 2 PQ0138 Total score: 104.62 Peptides matched: 2 Q27031 Total score: 104.62 Peptides matched: 2 Q43532 Total score: 104.62 Peptides matched: 2 Q93146 Total score: 104.62 Peptides matched: 2 Q93147 Total score: 104.62 Peptides matched: 2 Q94614 Total score: 104.62 Peptides matched: 2 Q95LN9 Total score: 104.62 Peptides matched: 2 Q9GPK0 Total score: 104.62 Peptides matched: 2 Q9GRE8 Total score: 104.62 Peptides matched: 2 Q9H3R6 Total score: 104.62 Peptides matched: 2 Q9M4E6 Total score: 104.62 Peptides matched: 2 Q9M6R1 Total score: 104.62 Peptides matched: 2 Q9NCC8 Total score: 104.62 Peptides matched: 2 Q9NGK9 Total score: 104.62 Peptides matched: 2 Q9NJB7 Total score: 104.62 Peptides matched: 2 Q9SAU8 Total score: 104.62 Peptides matched: 2 Q9TW75 Total score: 104.62 Peptides matched: 2 Q9U545 Total score: 104.62 Peptides matched: 2 Q9UAE9 Total score: 104.62 Peptides matched: 2 S07197 Total score: 104.62 Peptides matched: 2 S11456 Total score: 104.62 Peptides matched: 2 S27004 Total score: 104.62 Peptides matched: 2 S31716 Total score: 104.62 Peptides matched: 2 S36753 Total score: 104.62 Peptides matched: 2 S37165 Total score: 104.62 Peptides matched: 2 T21394 Total score: 104.62 Peptides matched: 2 T45478 Total score: 104.62 Peptides matched: 2 T45479 Total score: 104.62 Peptides matched: 2 A55719 Total score: 101.517 Peptides matched: 2 AAA37859 Total score: 101.517 Peptides matched: 2 AAD11466 Total score: 101.517 Peptides matched: 2 B36590 Total score: 101.517 Peptides matched: 2 B86295 Total score: 101.517 Peptides matched: 2 CAA33735 Total score: 101.517 Peptides matched: 2 HS74_YEAST Total score: 101.517 Peptides matched: 2 Q99KD7 Total score: 101.517 Peptides matched: 2 Q9TUG3 Total score: 101.517 Peptides matched: 2 Q9TX61 Total score: 101.517 Peptides matched: 2 S08211 Total score: 101.517 Peptides matched: 2 S10859 Total score: 101.517 Peptides matched: 2 AAA49563 Total score: 95.12 Peptides matched: 1 1HJOA Total score: 74.55 Peptides matched: 1 1NGA Total score: 74.55 Peptides matched: 1 1QQMA Total score: 74.55 Peptides matched: 1 1QQNA Total score: 74.55 Peptides matched: 1 1QQOA Total score: 74.55 Peptides matched: 1 A25773 Total score: 74.55 Peptides matched: 1 A29160 Total score: 74.55 Peptides matched: 1 A45871 Total score: 74.55 Peptides matched: 1 A48872 Total score: 74.55 Peptides matched: 1 A49242 Total score: 74.55 Peptides matched: 1 AAA03451 Total score: 74.55 Peptides matched: 1 AAA17441 Total score: 74.55 Peptides matched: 1 AAA57233 Total score: 74.55 Peptides matched: 1 AAA78276 Total score: 74.55 Peptides matched: 1 AAB03704 Total score: 74.55 Peptides matched: 1 AAD21815 Total score: 74.55 Peptides matched: 1 AAD21816 Total score: 74.55 Peptides matched: 1 AAL34314 Total score: 74.55 Peptides matched: 1 BAB72167 Total score: 74.55 Peptides matched: 1 BAB72168 Total score: 74.55 Peptides matched: 1 BAB72233 Total score: 74.55 Peptides matched: 1 BAB78505 Total score: 74.55 Peptides matched: 1 CAA52328 Total score: 74.55 Peptides matched: 1 E980236 Total score: 74.55 Peptides matched: 1 HS71_SCHPO Total score: 74.55 Peptides matched: 1 I37564 Total score: 74.55 Peptides matched: 1 I51129 Total score: 74.55 Peptides matched: 1 I54542 Total score: 74.55 Peptides matched: 1 JH0095 Total score: 74.55 Peptides matched: 1 JU0164 Total score: 74.55 Peptides matched: 1 O18474 Total score: 74.55 Peptides matched: 1 O73922 Total score: 74.55 Peptides matched: 1 PC1156 Total score: 74.55 Peptides matched: 1 Q26357 Total score: 74.55 Peptides matched: 1 Q26936 Total score: 74.55 Peptides matched: 1 Q26937 Total score: 74.55 Peptides matched: 1 Q63256 Total score: 74.55 Peptides matched: 1 Q63718 Total score: 74.55 Peptides matched: 1 Q98896 Total score: 74.55 Peptides matched: 1 Q98899 Total score: 74.55 Peptides matched: 1 Q98900 Total score: 74.55 Peptides matched: 1 Q98901 Total score: 74.55 Peptides matched: 1 Q9I8F9 Total score: 74.55 Peptides matched: 1 Q9IAC1 Total score: 74.55 Peptides matched: 1 Q9LU13 Total score: 74.55 Peptides matched: 1 Q9N1U2 Total score: 74.55 Peptides matched: 1 Q9NFT1 Total score: 74.55 Peptides matched: 1 Q9QWJ5 Total score: 74.55 Peptides matched: 1 S09036 Total score: 74.55 Peptides matched: 1 S25585 Total score: 74.55 Peptides matched: 1 S31766 Total score: 74.55 Peptides matched: 1 S37394 Total score: 74.55 Peptides matched: 1 S53357 Total score: 74.55 Peptides matched: 1 S67431 Total score: 74.55 Peptides matched: 1 T19211 Total score: 74.55 Peptides matched: 1 T22169 Total score: 74.55 Peptides matched: 1 T41121 Total score: 74.55 Peptides matched: 1 T43724 Total score: 74.55 Peptides matched: 1 T45468 Total score: 74.55 Peptides matched: 1 JQ1515 Total score: 71.4471 Peptides matched: 1 PC7036 Total score: 71.4471 Peptides matched: 1 Q9UVM0 Total score: 71.4471 Peptides matched: 1 A25089 Total score: 30.07 Peptides matched: 1 A32475 Total score: 30.07 Peptides matched: 1 A42582 Total score: 30.07 Peptides matched: 1 A48469 Total score: 30.07 Peptides matched: 1 AAA28075 Total score: 30.07 Peptides matched: 1 AAA34139 Total score: 30.07 Peptides matched: 1 AAA65099 Total score: 30.07 Peptides matched: 1 AAA92743 Total score: 30.07 Peptides matched: 1 AAA99920 Total score: 30.07 Peptides matched: 1 AAB06397 Total score: 30.07 Peptides matched: 1 AAB53051 Total score: 30.07 Peptides matched: 1 AAB63968 Total score: 30.07 Peptides matched: 1 AAF88019 Total score: 30.07 Peptides matched: 1 BAA13948 Total score: 30.07 Peptides matched: 1 BAB08435 Total score: 30.07 Peptides matched: 1 CAA02784 Total score: 30.07 Peptides matched: 1 CAA27330 Total score: 30.07 Peptides matched: 1 CAA28976 Total score: 30.07 Peptides matched: 1 CAA31393 Total score: 30.07 Peptides matched: 1 CAA31663 Total score: 30.07 Peptides matched: 1 CAA54419 Total score: 30.07 Peptides matched: 1 CAC84456 Total score: 30.07 Peptides matched: 1 D44261 Total score: 30.07 Peptides matched: 1 D90093 Total score: 30.07 Peptides matched: 1 H96605 Total score: 30.07 Peptides matched: 1 HHBYA1 Total score: 30.07 Peptides matched: 1 HS71_CANAL Total score: 30.07 Peptides matched: 1 HS71_PICAN Total score: 30.07 Peptides matched: 1 HS71_YEAST Total score: 30.07 Peptides matched: 1 HS72_PICAN Total score: 30.07 Peptides matched: 1 HS72_YEAST Total score: 30.07 Peptides matched: 1 HS7E_SPIOL Total score: 30.07 Peptides matched: 1 JC7132 Total score: 30.07 Peptides matched: 1 JQ0966 Total score: 30.07 Peptides matched: 1 O45038 Total score: 30.07 Peptides matched: 1 O48563 Total score: 30.07 Peptides matched: 1 O62564 Total score: 30.07 Peptides matched: 1 O65171 Total score: 30.07 Peptides matched: 1 O76306 Total score: 30.07 Peptides matched: 1 O93935 Total score: 30.07 Peptides matched: 1 O94104 Total score: 30.07 Peptides matched: 1 O94106 Total score: 30.07 Peptides matched: 1 P90655 Total score: 30.07 Peptides matched: 1 P91738 Total score: 30.07 Peptides matched: 1 P97966 Total score: 30.07 Peptides matched: 1 Q05829 Total score: 30.07 Peptides matched: 1 Q17310 Total score: 30.07 Peptides matched: 1 Q23712 Total score: 30.07 Peptides matched: 1 Q24895 Total score: 30.07 Peptides matched: 1 Q24928 Total score: 30.07 Peptides matched: 1 Q24952 Total score: 30.07 Peptides matched: 1 Q27078 Total score: 30.07 Peptides matched: 1 Q27121 Total score: 30.07 Peptides matched: 1 Q27146 Total score: 30.07 Peptides matched: 1 Q27147 Total score: 30.07 Peptides matched: 1 Q40924 Total score: 30.07 Peptides matched: 1 Q91624 Total score: 30.07 Peptides matched: 1 Q943K7 Total score: 30.07 Peptides matched: 1 Q94439 Total score: 30.07 Peptides matched: 1 Q961X6 Total score: 30.07 Peptides matched: 1 Q96IS6 Total score: 30.07 Peptides matched: 1 Q96W30 Total score: 30.07 Peptides matched: 1 Q9ATB8 Total score: 30.07 Peptides matched: 1 Q9DC27 Total score: 30.07 Peptides matched: 1 Q9FSY7 Total score: 30.07 Peptides matched: 1 Q9GPM5 Total score: 30.07 Peptides matched: 1 Q9GTX3 Total score: 30.07 Peptides matched: 1 Q9LHA8 Total score: 30.07 Peptides matched: 1 Q9M4E7 Total score: 30.07 Peptides matched: 1 Q9M4E8 Total score: 30.07 Peptides matched: 1 Q9NAX9 Total score: 30.07 Peptides matched: 1 Q9NCC9 Total score: 30.07 Peptides matched: 1 Q9NCD0 Total score: 30.07 Peptides matched: 1 Q9NCD1 Total score: 30.07 Peptides matched: 1 Q9NCD2 Total score: 30.07 Peptides matched: 1 Q9NCD3 Total score: 30.07 Peptides matched: 1 Q9NCD4 Total score: 30.07 Peptides matched: 1 Q9NCD5 Total score: 30.07 Peptides matched: 1 Q9NCD6 Total score: 30.07 Peptides matched: 1 Q9NCD7 Total score: 30.07 Peptides matched: 1 Q9NCD8 Total score: 30.07 Peptides matched: 1 Q9NCD9 Total score: 30.07 Peptides matched: 1 Q9NCE0 Total score: 30.07 Peptides matched: 1 Q9NHB4 Total score: 30.07 Peptides matched: 1 Q9P8E0 Total score: 30.07 Peptides matched: 1 Q9U540 Total score: 30.07 Peptides matched: 1 Q9ZS55 Total score: 30.07 Peptides matched: 1 S03250 Total score: 30.07 Peptides matched: 1 S14949 Total score: 30.07 Peptides matched: 1 S14950 Total score: 30.07 Peptides matched: 1 S18181 Total score: 30.07 Peptides matched: 1 S20139 Total score: 30.07 Peptides matched: 1 S21879 Total score: 30.07 Peptides matched: 1 S21880 Total score: 30.07 Peptides matched: 1 S24782 Total score: 30.07 Peptides matched: 1 S41372 Total score: 30.07 Peptides matched: 1 S42488 Total score: 30.07 Peptides matched: 1 S44168 Total score: 30.07 Peptides matched: 1 S46302 Total score: 30.07 Peptides matched: 1 S49303 Total score: 30.07 Peptides matched: 1 S51682 Total score: 30.07 Peptides matched: 1 S51712 Total score: 30.07 Peptides matched: 1 S53126 Total score: 30.07 Peptides matched: 1 S71171 Total score: 30.07 Peptides matched: 1 T03581 Total score: 30.07 Peptides matched: 1 T04078 Total score: 30.07 Peptides matched: 1 T04080 Total score: 30.07 Peptides matched: 1 T06358 Total score: 30.07 Peptides matched: 1 T06598 Total score: 30.07 Peptides matched: 1 T15513 Total score: 30.07 Peptides matched: 1 T34037 Total score: 30.07 Peptides matched: 1 T45298 Total score: 30.07 Peptides matched: 1 T45474 Total score: 30.07 Peptides matched: 1 T45475 Total score: 30.07 Peptides matched: 1 T45477 Total score: 30.07 Peptides matched: 1 T45517 Total score: 30.07 Peptides matched: 1 T45522 Total score: 30.07 Peptides matched: 1 T46574 Total score: 30.07 Peptides matched: 1 T46650 Total score: 30.07 Peptides matched: 1 T48270 Total score: 30.07 Peptides matched: 1 T48271 Total score: 30.07 Peptides matched: 1 AAB08760 Total score: 26.9671 Peptides matched: 1 AAC05418 Total score: 26.9671 Peptides matched: 1 AAF23276 Total score: 26.9671 Peptides matched: 1 P93937 Total score: 26.9671 Peptides matched: 1 Q91688 Total score: 26.9671 Peptides matched: 1 Q9NCC5 Total score: 26.9671 Peptides matched: 1 Q9NCC6 Total score: 26.9671 Peptides matched: 1 Q9NCC7 Total score: 26.9671 Peptides matched: 1 Protein Hit 2 =================== Accession : S14992 Description : dnaK-type molecular chaperone hsp70 - soybean Score : 166.04 Mass : 78349.7 Frame : 0 Coverage : 45 -- dim 2 808.3 1 (1 )TTPSYVAFTDTER 74.55 -- dim 3 973.9 2 (2 )NQVAMNPQNTVFDAK 61.42 -- dim 4 1084.9 3 (3 )IINEPTAAAIAYGLDKK 30.07 Proteins matching the same set of peptides: S53498 Total score: 166.04 Proteins matching a subset of these peptides: AAA74906 Total score: 135.97 Peptides matched: 2 B45871 Total score: 135.97 Peptides matched: 2 HHXL70 Total score: 135.97 Peptides matched: 2 I49761 Total score: 135.97 Peptides matched: 2 O75634 Total score: 135.97 Peptides matched: 2 O88686 Total score: 135.97 Peptides matched: 2 Q96QC9 Total score: 135.97 Peptides matched: 2 Q9R2A1 Total score: 135.97 Peptides matched: 2 S21365 Total score: 135.97 Peptides matched: 2 S41415 Total score: 135.97 Peptides matched: 2 AAA27868 Total score: 61.42 Peptides matched: 1 Q17228 Total score: 61.42 Peptides matched: 1 Q17289 Total score: 61.42 Peptides matched: 1 Q27379 Total score: 61.42 Peptides matched: 1 A34041 Total score: 58.3171 Peptides matched: 1 Protein Hit 3 =================== Accession : A36333 Description : dnaK-type molecular chaperone Hsc70-4 - fruit fly (Drosophila melanogaster) Score : 147.45 Mass : 77814.5 Frame : 0 Coverage : 45 -- dim 2 808.3 1 (1 )TTPSYVAFTDTER 74.55 -- dim 3 973.9 3 (3 )NQVAMNPTQTIFDAK 42.83 -- dim 4 1084.9 3 (3 )IINEPTAAAIAYGLDKK 30.07 Proteins matching the same set of peptides: AAF55150 Total score: 147.45 Proteins matching a subset of these peptides: Protein Hit 4 =================== Accession : A03309 Description : dnaK-type molecular chaperone Dsim/Hsc70-1 - fruit fly (Drosophila simulans) (fragments) Score : 147.03 Mass : 25558.9 Frame : 0 Coverage : 28 -- dim 2 808.3 3 (3 )TTPSYVAFTESER 57.19 -- dim 3 973.9 1 (1 )NQVAMNPNNTIFDAK 89.84 Proteins matching the same set of peptides: AAA28632 Total score: 147.03 JN0668 Total score: 143.927 Proteins matching a subset of these peptides: B03309 Total score: 57.19 Peptides matched: 1 Q9BIS1 Total score: 57.19 Peptides matched: 1 Q9BIS3 Total score: 57.19 Peptides matched: 1 Protein Hit 5 =================== Accession : Q95PU3 Description : HEAT SHOCK PROTEIN (HSP70).- Euplotes crassus. Score : 113.66 Mass : 80429.7 Frame : 0 Coverage : 30 -- dim 2 808.3 1 (1 )TTPSYVAFTDTER 74.55 CB dim 4 1084.9 1 (1 )IIIEPTAAAIAYGLDKK 39.11 Proteins matching the same set of peptides: Proteins matching a subset of these peptides: Protein Hit 6 =================== Accession : 1NGB Description : Heat-shock cognate 70kd protein (44kd atpase n-terminal Fragment) (EC 3.6.1.3) mutant with glu 175 replace d by Gln (e175q) - bovine Score : 109.89 Mass : 45220.2 Frame : 0 Coverage : 30 -- dim 2 808.3 1 (1 )TTPSYVAFTDTER 74.55 -- dim 4 1084.9 2 (2 )IINQPTAAAIAYGLDKK 35.34 Proteins matching the same set of peptides: Proteins matching a subset of these peptides: Protein Hit 7 =================== Accession : O61226 Description : Score : 90.71 Mass : 0 Frame : 0 Coverage : 30 -- dim 2 808.3 2 (2 )TTPSYVAFTNTER 60.64 -- dim 4 1084.9 3 (3 )IINEPTAAAIAYGLDKK 30.07 Proteins matching the same set of peptides: Proteins matching a subset of these peptides: Protein Hit 8 =================== Accession : P87036 Description : Score : 81.27 Mass : 0 Frame : 0 Coverage : 29 -- dim 1 671.9 3 (3 )DAGTIAGLEVLR 51.2 -- dim 4 1084.9 3 (3 )IINEPTAAAIAYGLDKK 30.07 Proteins matching the same set of peptides: Proteins matching a subset of these peptides: A25398 Total score: 51.2 Peptides matched: 1 AAA30204 Total score: 51.2 Peptides matched: 1 AG2111 Total score: 51.2 Peptides matched: 1 CAB59514 Total score: 51.2 Peptides matched: 1 O76958 Total score: 51.2 Peptides matched: 1 S06158 Total score: 51.2 Peptides matched: 1 S74372 Total score: 51.2 Peptides matched: 1 A45515 Total score: 48.0971 Peptides matched: 1 CAA36551 Total score: 48.0971 Peptides matched: 1 Q94594 Total score: 48.0971 Peptides matched: 1 Q9GYW3 Total score: 48.0971 Peptides matched: 1 Q9HG01 Total score: 48.0971 Peptides matched: 1 S11448 Total score: 48.0971 Peptides matched: 1 S52727 Total score: 48.0971 Peptides matched: 1 Score Delta Hit Protein Peptide Accessions that matched query 97 rank 1:- */
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