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Search title | : | 8plex iTRAQ from Inst. of Cancer Sciences, Univ. Manchester |
Databases | : | 1: contaminants 20090624 (262 sequences; 133,770 residues) 2: SwissProt 2019_11 (561,568 sequences; 201,997,950 residues) |
Taxonomy | : | 1: (none) 2: Homo sapiens (human) (20,368 sequences) |
Timestamp | : | 9 Jan 2020 at 11:48:02 GMT |
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Type of search | : | MS/MS Ion Search |
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Quantitation | : | iTRAQ 8plex (Applied Biosystems iTRAQ(TM) 8-plex) |
Enzyme | : | Trypsin/P |
Fixed modifications | : | Methylthio (C), iTRAQ8plex (N-term), iTRAQ8plex (K) |
Variable modifications | : | Deamidated (NQ), Oxidation (M), Oxidation (HW), Acetyl (Protein N-term), iTRAQ8plex (Y) |
Mass values | : | Monoisotopic |
Protein mass | : | Unrestricted |
Peptide mass tolerance | : | ± 20 ppm |
Fragment mass tolerance | : | ± 0.3 Da |
Max missed cleavages | : | 1 |
Instrument type | : | ESI-QUAD-TOF |
Number of queries | : | 184,516 |
Modification | Delta | Type | Site | Total matches |
---|---|---|---|---|
iTRAQ8plex | 304.20536 | fixed | N-term | 43505 |
iTRAQ8plex | 304.20536 | fixed | K | 22023 |
Methylthio | 45.987721 | fixed | C | 11377 |
iTRAQ8plex | 304.20536 | variable | Y | 4662 |
Deamidated | 0.984016 | variable | N | 4469 |
Oxidation | 15.994915 | variable | M | 2854 |
Oxidation | 15.994915 | variable | W | 445 |
Deamidated | 0.984016 | variable | Q | 190 |
Oxidation | 15.994915 | variable | H | 167 |
Acetyl | 42.010565 | variable | Protein N-term | 33 |
Dupes | … | Expect | Rank | U | 1 | 2 | Peptide | |
---|---|---|---|---|---|---|---|---|
0.037 | 2 | GAYSLSLR | significant | |||||
9 | 1 | GFFLFVEGGR | top ranking | |||||
6.4e-05 | 1 | GSSIFGLAPGK | significant and top ranking | |||||
1.3e-06 | 1 | SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||||
6.2e-07 | 1 | VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||||
6.4e-05 | 1 | U | GSSIFGLAPGK | unique | ||||
2 | 5.7e-05 | 1 | LNTLETEEWFFK | peptide has two duplicates | ||||
0.18 | 1 | LNTLETEEWFFK | duplicate peptide |
Score | … | 114/113 | 115/113 | ||
---|---|---|---|---|---|
CFAH_HUMAN | 37559 | 0.962 | 1.129 | ||
FHR2_HUMAN | 1330 | 0.859 | 1.128 |
Target | Decoy | FDR | ||||
---|---|---|---|---|---|---|
Protein family members | 545 | 191 | 35.05% | |||
36368 | 290 | 0.80% | ||||
Target | Decoy | FDR | ||||
Protein family members | 545 | 191 | 35.05% | |||
43585 | 600 | 1.38% | ||||
Target | Decoy | FDR | ||||
Protein family members | 545 | 191 | 35.05% | |||
2882 | 74 | 2.57% | ||||
Target | Decoy | FDR | ||||
Protein family members | 545 | 191 | 35.05% | |||
3310 | 200 | 6.04% |
Decoy results are available in the decoy report.
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::CO4B_HUMAN | 164342 | Complement C4-B OS=Homo sapiens OX=9606 GN=C4B PE=1 SV=2 | |
2 | 2::CO4A_HUMAN | 163856 | Complement C4-A OS=Homo sapiens OX=9606 GN=C4A PE=1 SV=2 | |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::APOB_HUMAN | 127385 | Apolipoprotein B-100 OS=Homo sapiens OX=9606 GN=APOB PE=1 SV=2 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::CERU_HUMAN | 59576 | Ceruloplasmin OS=Homo sapiens OX=9606 GN=CP PE=1 SV=1 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::A1BG_HUMAN | 58870 | Alpha-1B-glycoprotein OS=Homo sapiens OX=9606 GN=A1BG PE=1 SV=4 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::HEMO_HUMAN | 44576 | Hemopexin OS=Homo sapiens OX=9606 GN=HPX PE=1 SV=2 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::CFAH_HUMAN | 37520 | Complement factor H OS=Homo sapiens OX=9606 GN=CFH PE=1 SV=4 | |
2 | 2::FHR2_HUMAN | 1329 | Complement factor H-related protein 2 OS=Homo sapiens OX=9606 GN=CFHR2 PE=1 SV=1 | |
3 | 2::FHR1_HUMAN | 1289 | Complement factor H-related protein 1 OS=Homo sapiens OX=9606 GN=CFHR1 PE=1 SV=2 | |
4 | 2::FHR5_HUMAN | 699 | Complement factor H-related protein 5 OS=Homo sapiens OX=9606 GN=CFHR5 PE=1 SV=1 | |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::ITIH2_HUMAN | 35418 | Inter-alpha-trypsin inhibitor heavy chain H2 OS=Homo sapiens OX=9606 GN=ITIH2 PE=1 SV=2 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::FETUA_HUMAN | 35203 | Alpha-2-HS-glycoprotein OS=Homo sapiens OX=9606 GN=AHSG PE=1 SV=2 |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::CFAB_HUMAN | 31648 | Complement factor B OS=Homo sapiens OX=9606 GN=CFB PE=1 SV=2 | |
2 | 1::00718067.1 | 3489 | (Bos taurus) Complement factor B | |
Accession | Score | Description | ||
---|---|---|---|---|
1 | 2::APOH_HUMAN | 30084 | Beta-2-glycoprotein 1 OS=Homo sapiens OX=9606 GN=APOH PE=1 SV=3 |
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