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Posted by Ville Koskinen (January 18, 2019)

O-fucosylated CID spectra

O-linked fucose is easily lost in CID. A recent paper by Swearingen et al. in the Journal of Proteome Research discusses this in the context of identifying O-fucosylated thrombospondin type 1 repeats (TSRs) in Plasmodium parasites using database searching. The main problem is, the O-glycosidic bond is weaker than the peptide backbone. Collision energies typical for peptide fragmentation cause it [...]

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Posted by John Cottrell (September 19, 2013)

Modifications round-up, part 1

Much of the complexity in Mascot is associated with modifications. It can be hard to find information about some of aspects of handling modifications unless you already know what you are looking for. In this blog article, the first of two, I’ll collect together some of the topics that come up frequently in support emails. Note that Site analysis was [...]

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