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Mascot Training

Learn how to get the best out of Mascot directly from the people who develop and support it.

Course Announcements

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Course Outline

We have created an intensive 2-day course that covers every aspect of protein identification and characterisation using Mascot. Each course will be limited to 20 participants to ensure that there is ample opportunity for questions and interaction.
Day 1

Introduction

  • The three ways to search MS and MS/MS data
  • Practical tips
  • Why searches can fail

Search Parameters

  • A detailed look at each of the search parameters
  • Tips and common mistakes
  • Setting defaults

Sequence Databases

  • Which database to choose
  • Nucleic acid databases
  • Clustering with UniGene
  • Searching whole genomes

Modifications

  • Types of modifications
  • Unimod
  • Phosphorylation
  • Error tolerant searches

Scoring & Statistics

  • Different ways of finding a match
  • Probability based scoring
  • Result reports
  • Significance thresholds
  • Assigning peptide matches to proteins
  • Validation

Very Large Searches

  • Challenges of very large searches
  • MudPIT protein scoring
  • Exporting search results
Day 2

Mascot Distiller

  • What is Mascot Distiller?
  • Peak picking
  • Automation
  • Importing search results
  • Calling sequence tags
  • De novo sequencing

Sequence Queries

  • Standard sequence tag
  • Error tolerant sequence tag
  • seq(), comp() and other qualifiers

Mascot Daemon

  • Installing and using Mascot Daemon
  • Data import filters
  • Real-time monitor
  • Follow-up tasks

Quantitation

  • Supported protocols
  • Creating and modifying quantitation
    methods

Sequence Database Administration

  • Adding a new database
  • Worked examples
  • Automatic updating

General Mascot Server Administration

  • Configuring Mascot Security
  • Mascot configuration files
  • Mascot log files
  • Cluster mode

Course Schedule

2010
Location Venue Google Map Dates
Hannover, Germany
Fully booked
ANDOR Hotel Plaza
Fernroder Straße 9
30161 Hannover
Map 26 - 27 April 2010
Washington DC / Bethesda MD, USA
Fully booked
Bethesda North Marriott Hotel & Conference Center
5701 Marinelli Road
Bethesda, MD 20852
Map 20 - 21 May 2010
Shanghai, China 信息 和注册 (Information and Registration)   1 - 2 July 2010
Beijing, China 信息 和注册 (Information and Registration)   5 - 6 July 2010
London, UK
Fully booked
RIBA
66 Portland Place
London W1B 1AD
Map 2 - 3 September 2010

Language: All courses are taught in English.

Prerequisites: A working knowledge of protein chemistry and mass spectrometry is required to obtain maximum benefit from the course.

To participate in the exercises, you need to bring an Intel based laptop PC with a DVD drive and WiFi. The DVD drive will enable you to run a local Mascot server by booting to a LiveDVD. A WiFi Point of Presence will be available, allowing you to connect to the Matrix Science public Mascot server. Administrator rights will be required if you want to install Mascot Daemon and Mascot Distiller.

Programme: Teaching is from 10:00am to 5:00pm on day 1 and 9:00am to 4:00pm on day 2, with a 1 hour break for lunch. The instructors will be available at the end of each day for questions and discussion.

Course materials will be supplied as PDF files on a complementary USB memory stick. There will be no printed handouts. If you wish to make notes, please bring writing materials.

Registration and Payment

Course fees are $850 / €600 / £550 for academic & non-profit organisations, $1250 / €900 / £800 for commercial organisations. Delegates from UK organisations registering for a course in the EU (including the UK) must add 17.5% VAT or supply a VAT exemption certificate. Delegates from other EU countries do not need to add VAT as long as they supply a valid VAT registration number.

To register, print out this form and return it by fax, email or post. You can pay by credit card, cheque, purchase order or bank transfer; full details are on the form.

The number of attendees on each course is limited and places will be allocated as payment is received. If you cancel two months or more prior to the course start date, 90% of the fee will be refunded. One month or more, 60%. No refunds are possible for cancellations received within one month of the course start date. If you are unable to attend, you have the option of substituting someone else from your organisation.

FAQ

What is included in the cost of the course?

The course fee covers the teaching and hand-out materials, refreshments and lunch. It does not include other meals, accommodation, or travel.

Can you help with booking of travel or accommodation?

Sorry, no. You must make your own arrangements.

Are there discounts for multiple enrollments?

Multiple enrollments on a single course from a single organisation qualify for 10% discount

Can my organisation arrange for an in-house course?

Please contact us to discuss a course customised to your specific requirements.

Can I propose a location for a future course?

Please send requests for other dates and locations by email to training@matrixscience.com

What if I have a question that isn't covered anywhere on this page?

Please email training@matrixscience.com
 
 
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