Mascot Training
Learn how to get the best out of Mascot directly from the people who develop and support it.
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We have created an intensive 2-day course that covers every aspect of protein identification and
characterisation using Mascot. Each course will be limited to 20 participants to ensure that there is
ample opportunity for questions and interaction.
| Day 1
Introduction
- The three ways to search MS and MS/MS data
- Practical tips
- Why searches can fail
Search Parameters
- A detailed look at each of the search parameters
- Tips and common mistakes
- Setting defaults
Sequence Databases
- Which database to choose
- Nucleic acid databases
- Clustering with UniGene
- Searching whole genomes
Modifications
- Types of modifications
- Unimod
- Phosphorylation
- Error tolerant searches
Scoring & Statistics
- Different ways of finding a match
- Probability based scoring
- Result reports
- Significance thresholds
- Assigning peptide matches to proteins
- Validation
Very Large Searches
- Challenges of very large searches
- MudPIT protein scoring
- Exporting search results
|
Day 2
Mascot Distiller
- What is Mascot Distiller?
- Peak picking
- Automation
- Importing search results
- Calling sequence tags
- De novo sequencing
Sequence Queries
- Standard sequence tag
- Error tolerant sequence tag
- seq(), comp() and other qualifiers
Mascot Daemon
- Installing and using Mascot Daemon
- Data import filters
- Real-time monitor
- Follow-up tasks
Quantitation
- Supported protocols
- Creating and modifying quantitation
methods
Sequence Database Administration
- Adding a new database
- Worked examples
- Automatic updating
General Mascot Server Administration
- Configuring Mascot Security
- Mascot configuration files
- Mascot log files
- Cluster mode
|
| 2010 |
| Location |
Venue |
Google Map |
Dates |
Hannover, Germany Fully booked |
ANDOR Hotel Plaza
Fernroder Straße 9
30161 Hannover
|
Map |
26 - 27 April 2010 |
Washington DC / Bethesda MD, USA Fully booked |
Bethesda North Marriott Hotel & Conference Center
5701 Marinelli Road
Bethesda, MD 20852 |
Map |
20 - 21 May 2010 |
| Shanghai, China |
信息 和注册 (Information and Registration) |
|
1 - 2 July 2010 |
| Beijing, China |
信息 和注册 (Information and Registration) |
|
5 - 6 July 2010 |
London, UK Fully booked |
RIBA
66 Portland Place
London W1B 1AD
|
Map |
2 - 3 September 2010 |
Language: All courses are taught in English.
Prerequisites: A working knowledge of protein chemistry and mass spectrometry is
required to obtain maximum benefit from the course.
To participate in the exercises, you need to bring an Intel based laptop PC with
a DVD drive and WiFi. The DVD drive will enable you to run a local Mascot server
by booting to a LiveDVD. A WiFi Point of Presence will be available, allowing you
to connect to the Matrix Science public Mascot server. Administrator rights will
be required if you want to install Mascot Daemon and Mascot Distiller.
Programme: Teaching is from 10:00am to 5:00pm on day 1 and 9:00am to 4:00pm on day 2,
with a 1 hour break for lunch. The instructors will be available at the end
of each day for questions and discussion.
Course materials will be supplied as PDF files on a complementary USB memory stick. There
will be no printed handouts. If you wish to make notes, please bring writing materials.
Course fees are $850 / €600 / £550 for academic & non-profit organisations,
$1250 / €900 / £800 for commercial organisations. Delegates from UK organisations
registering for a course in the EU (including the UK) must add 17.5% VAT or supply a VAT
exemption certificate. Delegates from other EU countries do not need to add VAT as long as
they supply a valid VAT registration number.
To register, print out this form
and return it by fax, email or post. You can pay by credit card, cheque, purchase order or
bank transfer; full details are on the form.
The number of attendees on each course is limited and places will be allocated
as payment is received. If you cancel two months or more prior to the course start date,
90% of the fee will be refunded. One month or more, 60%. No refunds are possible for
cancellations received within one month of the course start date. If you are unable
to attend, you have the option of substituting someone else from your organisation.
What is included in the cost of the course?
The course fee covers the teaching and hand-out materials, refreshments and lunch. It does not
include other meals, accommodation, or travel.
Can you help with booking of travel or accommodation?
Sorry, no. You must make your own arrangements.
Are there discounts for multiple enrollments?
Multiple enrollments on a single course from a single organisation
qualify for 10% discount
Can my organisation arrange for an in-house course?
Please contact us to discuss a course
customised to your specific requirements.
Can I propose a location for a future course?
Please send requests for other dates and locations by email to
training@matrixscience.com
What if I have a question that isn't covered anywhere on this page?
Please email training@matrixscience.com
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