Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

Blog

Articles tagged: Proteome Discoverer

Return to blog archive

Posted by Ville Koskinen (May 29, 2025)

Identify more proteins using Mascot and Proteome Discoverer with this one small trick

Mascot Server is often used with software packages like Thermo Proteome Discoverer™ (PD). We recently made it possible to enable machine learning on the server side by specifying it in the Mascot instrument definition, which is also the topic of one of our ASMS 2025 posters. An important detail was overlooked in the initial instructions, which is: you should disable [...]

Full article and comments form

Posted by Ville Koskinen (January 31, 2025)

Using machine learning with Mascot and Proteome Discoverer

Update: There is a newer version of this article, Identify more proteins using Mascot and Proteome Discoverer with this one small trick. The PD setup instructions have been updated accordingly. Mascot Server 3.1 is a patch release that enhances integration with Thermo Proteome Discoverer™ and fixes a few important bugs introduced in Mascot Server 3.0. The full list of improvements [...]

Full article and comments form

Posted by John Cottrell (April 15, 2019)

Mascot workflows in Proteome Discoverer

For many users of Thermo instruments, Proteome Discoverer (PD) is their primary user interface for database searching, and Mascot is represented by a node in the workflow. This article collects together a few tips and observations concerning Proteome Discoverer 2.3 and Mascot Server 2.6. Proteome Discoverer Configuration Under Administration; Mascot Server, the setting Max. MGF File Size [MB] has a [...]

Full article and comments form