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Articles tagged: timsTOF
Top-3 Quantitation Summary in Mascot Daemon 3 using DDA-PASEF
Mascot Daemon includes a protein level quantitation summary report for both precursor based methods (LFQ, SILAC etc) and MS/MS quantitation (iTRAQ, TMT). The exported format is suitable for importing into statistic packages such as R or Perseus. In Mascot Daemon 3, we added an option to export protein intensity values calculated using the “Top-3″ method. Instead of using all matched [...]
Improving precursor quantitation results with ion mobility filtering
One of the major new features in Mascot Distiller 2.8 is support for raw data files from the Bruker timsTOF instrument. timsTOF combines an ion mobility dimension with QTOF, allowing an additional separation dimension. The instrument can use this to help separate MS/MS spectra from otherwise isobaric peptides. Mascot Distiller 2.8 can use the precursor ion mobility values for identified [...]