Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

Exercise ADM2: hints

Mascot 2.4 and later

  • Launch Database Manager
  • Create New
  • Enter suitable name and choose Use predefined definition template
  • Select Simple NA template
  • Create
  • Copy Fasta file to specified directory and choose Refresh
  • Activate

Mascot 2.3 and earlier

  • Create a directory on the Mascot server for the Fasta file, e.g. C:\inetpub\mascot\sequence\h_pylori\current or /usr/local/mascot/sequence/h_pylori/current
  • Copy the Fasta file to the directory
  • Launch Database Maintenance
  • Choose New Definition
  • Configure similar to this


  • Test and, if all OK, Apply


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