Exercise PMF4
Topic
To investigate how PMF mixtures are reported
Requirements
User access to any Mascot server, including the Matrix Science public web site
Experimental
MALDI-TOF data from a 2D gel spot. Protein(s) are from yeast. Enzyme trypsin, state of cysteine unimportant, mass tolerance 100 ppm. The data file contains a list of MH+ values
Data
Right-click this link: PMF4.txt, and choose Save target as or Save link as
Tips
- Try searching SwissProt and NCBInr with and without a taxonomy filter
Questions
- What are the effects of database size?
- How many components are you confident are present?
- Does the search engine report a choice of mixtures? If so, what does this mean?
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