Posted by Web Master (June 10, 2004)

Mascot support for Electron Transfer Dissociation

At this year’s ASMS in Nashville, there was much interest in Electron Transfer Dissociation (ETD) for phosphopeptide analysis, (abstract). ETD, which generates c and z fragmentation, is very similar to Electron Capture Dissociation (ECD). Although the authors were interpreting their spectra manually, you can readily search them using Mascot. Choose FTMS-ECD as the INSTRUMENT, which selects c, z, and z+H ion series. If you are looking for phosphorylated peptides, choose modifications without neutral loss: Phospho-NL (S), Phospho-NL (T), and Phospho (Y). The first two are "hidden" modifications. You may need to change your search form defaults to enable their display in the search form. (Follow the hyperlink on the Search form selection page.)

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