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Posted by Richard Jacob (November 16, 2017)

Using a shared TaskDB with Mascot Daemon

Mascot Daemon is our automation client for Mascot Server. The client was introduced with Mascot Server version 1.6 and has been continually developed ever since. The licensing for Mascot Daemon allows as many copies of Daemon to be installed in your lab as you like. By default, each copy of Daemon uses its own task database, TaskDB, to store information [...]

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Posted by John Cottrell (February 14, 2015)

Searching DIA data, especially Swath

DIA-Umpire is a new, open source Java program that enables untargeted peptide and protein identification and quantitation using DIA data. A detailed description can be found in Nature Methods. The DIA-Umpire signal extraction module deconvolutes the DIA data to create a conventional DDA-type peak list, suitable for database searching. The software is intended to be applicable to DIA from any [...]

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Posted by John Cottrell (February 1, 2013)

Don’t get stuck in a queue

Mascot Server doesn’t queue searches. This isn’t because we can’t be bothered to write the code. Its because we don’t believe queuing would improve performance or usability. The times taken for individual searches cover a very wide range. A PMF or a small MS/MS search with few variable modifications and tryptic specificity might take seconds. A large MS/MS search of [...]

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