Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

Exercise SQ3


Generate an error tolerant tag search from a de novo solution


User access to any Mascot server, including the Matrix Science public web site


The starting point for this exercise is the Distiller project you saved at the end of exercise DIS2. Perform error tolerant tag searches of the top scoring de novo solutions.


  1. Can you get a convincing match and can you explain the mass difference between the database sequence and the analyte?


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