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Posted by Patrick Emery (January 16, 2017)

How many of you are there in there? Processing and searching chimeric MS/MS spectra with Mascot Distiller and Mascot Server

In the typical shotgun proteomics experiment, the assumption is that each MS/MS spectrum is derived from a single precursor selected by the Mass Spectrometer for fragmentation. However, in practice, near isobaric precursors can co-elute and undergo co-fragmentation resulting in chimeric MS/MS spectra containing fragments from multiple different precursor peptides. With high resolution data, it is possible for these overlapping isotope [...]

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