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Posted by John Cottrell (June 14, 2019)

What are you inferring?

Benchmarking protein inference is notoriously difficult. Artificial samples of known content tend to be too simple while real samples lack ground truth. An interesting approach was adopted for the ABRF iPRG 2016 study, and has been the subject of a publication from The et al. A collection of human Protein Epitope Signature Tags (PrESTs) were expressed in E. coli and [...]

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Posted by Ville Koskinen (April 13, 2018)

Keeping genome databases up to date

Database Manager is a great tool for keeping your sequence databases up to date in Mascot. If the database is available as a ready-made FASTA file, all you need to do is enable it as a predefined definition, or set up a definition to download the file from a known URL (see the help for more details). Updating the database [...]

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Posted by John Cottrell (September 12, 2017)

How to create a spectral library for contaminants

An earlier article highlighted how modified and non-specific peptides from contaminants can be matched using a spectral library without increasing the search space for the target proteins. This is particularly useful for sequencing grade trypsin, which is modified by methylation or acetylation of the lysines, creating a large number of modified non-specific peptides that are missed by typical search strategies. [...]

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Posted by John Cottrell (January 14, 2016)

Disruption ahead for NCBI databases

NCBI has announced that it will drop ‘gi number’ unique identifiers on June 15, 2016. Details are given in section 1.4 of the GenBank release notes. This will create difficulties for users of many bioinformatic tools, not just Mascot. Particularly in the context of major projects, where analyses are performed over an extended time period and results consolidated by protein [...]

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