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Posted by Patrick Emery (April 28, 2021)

Global thinking: Label Free Quantitation in Mascot Distiller 2.8

Mascot Distiller supports a wide range of precursor based quantitation methods, including two label-free methods, which we call Replicate and Average. Version 2.8 makes significant improvements to the Replicate method. This is the method for label-free quantitation based on the relative intensities of extracted ion chromatograms (XICs) for precursors in multiple data sets aligned using mass and elution time. Replicate [...]

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Posted by John Cottrell (October 19, 2016)

Retention time

Although retention time is not part of the Mascot scoring algorithm, it can be used by Percolator to improve the re-scoring of search results and it is important or essential information for many types of quantitation. This article examines how retention time is represented in the peak list, the search results, and various export formats. Mascot Server works off a [...]

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