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Archive
Date | Topics | |
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September 2024 | • Machine learning in Mascot Server 3.0 • Untargeted identification of viruses • Preorder Mascot 3.0 now |
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August 2024 | • 30 years of Sequence Tags • Proteomics of peanut allergens • Error tolerant sequence tag example |
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July 2024 | • Mascot workflow for LC-MS/MS • Adaptive epigenetic changes in histone variants • Free temporary licence for migration |
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June 2024 | • Breakfast meeting summary • Mascot at ASMS 2024 |
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May 2024 | • ASMS User Meeting • Optimizing sample preparation with an artificial neural network • Free Mascot service |
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April 2024 | • ASMS User Meeting • Wind effect in plant protein expression • New distributor in Europe |
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March 2024 | • Guest user settings • Impact of software on fast photochemical oxidation of protein (FPOP) analysis • Mascot for your research lab |
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February 2024 | • Unsuspected mods with DIA • Spatial heterogeneity of uterine cancer tumors • Deleting obsolete predefined definitions |
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January 2024 | • Narrow window DIA • Ubiquitylation and DNA damage response • Windows Server 2019 |
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December 2023 | • ABRF Crosslinking Study • N-terminal acetylation shields proteins from degradation • Internal fragments with HCD |
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November 2023 | • Chimeric spectra • Characterizing Shewanella integral membrane complex • Mascot Distiller 2.8.5 |
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October 2023 | • Distiller’s 20th anniversary • LAP-MALDI MS for detection of bovine tuberculosis • Automated Distiller reports |
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September 2023 | • Software version numbers • Mapping the human chondroitin sulfate glycoproteome • Mascot Server 2.8.3 |
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August 2023 | • Optimise your chunk size • Sequencing isomeric proteoforms using cyclic ion mobility and ECD • Mascot Distiller 2.8.4 |
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July 2023 | • Creating DIA spectral libraries • Mass spectral analyses of Salmonella myovirus • Crosslinking with DSSO |
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June 2023 | • User meeting slides • Mascot posters at ASMS • Updated training materials |
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May 2023 | • Breakfast meeting at ASMS • High-throughput venomics • Mascot in the Cloud |
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April 2023 | • Breakfast meeting at ASMS • Bioinertization of nanoLC/MS/MS systems for phosphoproteomics • 25 years of Matrix Science |
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March 2023 | • Crosslinked peak picking • Targeted protein dephosphorylation • Batch scripting with Distiller |
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February 2023 | • Paleoproteomics • Biomarkers in the exosome of endometrial cancer serum • Staying up to date |
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January 2023 | • Complementary reporter ions • Growing ryegrass on Martian regolith simulant • Free disk space |
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December 2022 | • Distiller quant reports • Tubulin glutamylation and glycylation • TMTpro quant methods |
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November 2022 | • Trusting your search engine • Temporal phosphoproteomics of EV-A71-infected cells • Virtualisation |
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October 2022 | • Fractionated LFQ • PMF of preserved collagen in archaeological fish bones • System limits on Linux |
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September 2022 | • mzIdentML 1.2 • Biomarkers of residual malaria parasites • Mascot Server 2.8.2 |
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August 2022 | • SARS-CoV-2 spike protein disulfide bonds • Protein products of non-coding transcripts • Disaster recovery |
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July 2022 | • Single-cell spectra • VAMS-Trypsin immobilization for instant proteolysis • New pricing for updating old licences |
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June 2022 | • ASMS presentations • Identification of Protein Isoforms using RNA-seq reads • False Discovery Rates |
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May 2022 | • ASMS User Meeting • Peak picking with Distiller • Androgen receptor interactions • Glycans in Unimod |
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April 2022 | • ASMS User Meeting • NCBI nr tips • Filter-aided sample preparation • Mascot Server patch |
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March 2022 | • Linux cluster security • Glycation in milk • EST Fasta files |
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February 2022 | • FTP retirement • Histone code of the malaria parasite • Mascot Distiller update |
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January 2022 | • Hardware for Mascot Distiller • Serological testing for SARS-CoV-2 • Making Protein Family Summary the default report |
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December 2021 | • Endogenous peptides • Solid phase extraction • Mascot Daemon housekeeping |
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November 2021 | • ASMS 2021 presentations • 3D bioprinted tumor models |
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October 2021 | • Disk bottlenecks • N-terminally ubiquitinated proteins • Local vs. public modifications |
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September 2021 | • Error tolerant search statistics • Anti-bacterial peptides • Exporting ion mobility values |
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August 2021 | • Ion mobility filtering • Proteomics of Primary Uveal Melanoma • Mascot Server 2.8 release |
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July 2021 | • LFQ with Mascot Distiller 2.8 • Mapping proximity associations • Retention time in Percolator |
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June 2021 | • Counterfeit cashmere • Protein carriers • Mascot Server 2.8 |
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May 2021 | • Unipept for taxonomy assignment • Extracellular matrix proteome • Mascot Distiller 2.8 |
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April 2021 | • Updated NIST Human spectral libraries • Histone methylation profiling • Standard enzyme definitions |
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March 2021 | • Sizing a Mascot Server license • Collagen in Ancient Bone • NCBI nr alternatives |
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February 2021 | • Single Sign-On • Zirconium-IMAC • Variable modification tuning |
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January 2021 | • de novo Competition Results • Automated plasma proteome preparation • Cross-link modification |
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December 2020 | • de novo Competition • Database management • ADP-ribosylated peptidesk • Spectral quality tutorial |
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November 2020 | • Crosslinked peptide ID • Enriching N-terminal peptides • Client error message quirk |
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October 2020 | • Quantitation charts and plots • Sialylation in rat nerve terminals • Custom and predefined database configurations |
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September 2020 | • Variable modifications • Aberrant N-glycosylation • Support and update reminder |
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August 2020 | • Summarizing quantitation data • Host cell proteins in biopharmaceuticals • Database Manager error message |
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July 2020 | • Human Proteome Project Guidelines • Cysteine quantitation • Creating new crosslinking methods |
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June 2020 | • ASMS Reboot Presentations • Quantitation p-values |
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May 2020 | • Remote working • Shotgun proteomics of SARS-CoV-2 • Finding help topics |
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April 2020 | • Protein FDR • N-term Arg and Lys enzyme • New databases on public server |
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March 2020 | • Disaster Recovery • In-line sample preparation • Spectral library creation |
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February 2020 | • Search reproducibility • Ion mobility separation of proteoforms • Modifications in spectral libraries |
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January 2020 | • Mascot Server 2.7 • mAb sequence validation • Uniprot proteomes |
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December 2019 | • Protein scores • Persulfidation • Access database engine |
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November 2019 | • Diethylation • Ionic liquid-based sample preparation • IIS configuration |
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October 2019 | • Shared peptides • Palmitoylation And Nitrosylation Interplay Monitoring • Fixed and variable modifications |
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September 2019 | • Benchmarking protein inference • Photosynthetic apparatus of Pinaceae • Controlling search priorities |
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August 2019 | • Peptide-spectrum match statistics • Epitope mapping by direct nano-electrospray MS • Windows versus Linux version of Mascot |
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July 2019 | • Proteome Discoverer workflows • Summed intensities improve iTRAQ accuracy • Neutral loss configuration |
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June 2019 | • Metaproteomics with Mascot • Middle-Down searching • Exclusive mods, combination mods, metabolic labelling |
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May 2019 | • ASMS User Meetings 2019 • Daemon automation • Analysis of C Terminome • Distiller quantitation speed-up |
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April 2019 | • ASMS User Meetings 2019 • X!Hunter spectral libraries • Identification of PEG-based detergents • Combining Mascot Server licences |
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March 2019 | • Proteomic Forum 2019 • O-glycosylated peptides • Adsorption-based sample preparation • Capturing error messages |
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February 2019 | • Use spectral libraries today • High-resolution spatial proteomics • Enzyme mixtures |
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January 2019 | • 1 Da modifications • Unexpected modifications • Using Report Builder |
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December 2018 | • Quantitation statistics • Single Amino Acid Variant discovery • De-charging a peak list |
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November 2018 | • Mascot Server cluster mode • Ion suppression in phosphoproteomics • Rebuilding the search log |
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October 2018 | • Common mistakes • Deep dive on human CSF • Troubleshooting corrupt XML |
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September 2018 | • Search parameter optimization • Identifying proteins in ancient paintings • NCBIprot maximum sequence length |
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August 2018 | • Free consultations at HUPO • Mascot Distiller speed • Fibronectin Proteolysis • Filtering out short peptides |
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July 2018 | • HUPO consultation sessions • Quantitation of cysteine oxidation • Catechol Estrogens • Editing the Mascot Daemon task database |
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June 2018 | • Genome database updates • Determination of site-specific phosphorylation ratios • Troubleshooting Mascot Daemon |
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May 2018 | • ASMS User Meetings • HTTPS Issues • Mining the human tissue proteome for protein citrullination • Mascot Server 2.6.2 |
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April 2018 | • 20-20 Special Offer • ASMS Workshops • MS3 Reporter Ions • Tip-based identification of protease substrates • Mascot Distiller 2.7 |
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March 2018 | • 25 years of protein ID • Glycopeptide identification • PC hardware specification |
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February 2018 | • Dark matter challenge results round-up • Biomarkers for Alzheimer’s • Model Distiller project |
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January 2018 | • Shared task database • Peptide biomarkers for ovarian cancer • UniProt reference proteome |
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December 2017 | • Alkylation issues • Detergents for Membrane Protein Complexes • Controlling running searches |
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November 2017 | • Contaminants spectral library • Non-canonical phosphorylation • Excluding modifications from error tolerant search |
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October 2017 | • Trypsin autolysis • Kinase activity ranking • Disabling cache files |
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September 2017 | • Which ions series? • SLIM labelling • Modifying the taxonomy tree |
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August 2017 | • Mascot security • Protein N-term characterization • Quantitation of extreme ratios |
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July 2017 | • Dark matter challenge • Alkylation side-effects • Anti-virus advice |
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June 2017 | • Exporting search results • Detection of methicillin-resistant S. aureus • Housekeeping automation |
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May 2017 | • ASMS breakfast meetings • Peak lists • Giant Buddhas • Reporting combined results |
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April 2017 | • ASMS breakfast meetings • Protein FDR • Protein carbonylation • Adding a new element |
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March 2017 | • Proteomics repositories • Trace-level residual proteins in human Ig • Processing options files in Mascot Distiller |
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February 2017 | • Chimeric spectra • Yeast small subunit processome • Initiator Met |
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January 2017 | • Retention Time in Mascot • The Protein Co-Migration Database • Why summary reports no longer show non-significant matches |
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December 2016 | • Spectral library searching • The epichaperome • Mascot Daemon 2.6 |
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November 2016 | • Hardware for Mascot Server • Proteasome-generated spliced peptides • Very long sequences |
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October 2016 | • Multiple fragmentation methods • Phosphoproteomics of Flu • Ambiguous residues |
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September 2016 | • High FDRs for methylated peptides • Protein primary structure of the Vaccinia virion • NCBI have dropped gi number identifiers |
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August 2016 | • HUPO 2016 • mzTab export • HLA-DR bound peptides • Negative ions |
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July 2016 | • Unlabelled peaks • Bacterial Interactomes • Peptide charge state |
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June 2016 | • Mascot Distiller 2.6 • Circadian clock proteins • Mysterious crashes |
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May 2016 | • ASMS breakfast meetings • Selenocysteine and U • Tetracycline-inhibited ribosome profiling • The database *.stats file |
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April 2016 | • ASMS breakfast meetings • ETD analysis of Histone Proteoforms • Web server timeout |
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March 2016 | • Search only for peptides we care about • Cryo-slicing Blue Native-MS • YouTube video |
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February 2016 | • Quality assurance • Histone PTMs • Command line search |
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January 2016 | • Listing confidently identified proteins • California sea lion CSF • Introductory tutorials |
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December 2015 | • Baker Street • Methionine Sulfoxide Dynamics • Distiller project viewer |
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November 2015 | • Mass-tolerant search • S-glutathionylation • CID+ETD Instrument |
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October 2015 | • De novo sequencing • Giant virus from permafrost • Intensity values for components |
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September 2015 | • Mascot Distiller • Microbial antigen typing • Customizing modifications |
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August 2015 | • Search with ESTs • Identification of Regulatory and Cargo Proteins • Mascot Security |
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July 2015 | • How do I do that? • Enrichment of Vesicular Glycoproteins • Webcast training course updated |
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June 2015 | • Mascot Server in "The Cloud" • Online Peptide Fractionation Using an LC-chip • Mascot tip – Auto-export in Mascot Daemon |
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May 2015 | • ASMS User Meeting • Site analysis • Global Analysis of Myocardial Peptides • Mascot tip – Glycopeptide analysis |
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April 2015 | • ASMS User Meeting • Mascot on a virtual machine • Phospho-iTRAQ: Assessing Isobaric Labels for the Large-Scale Study Of Phosphopeptide Stoichiometry • Mascot tip – Migrating to new hardware |
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March 2015 | • DIA-Umpire enables database searching of DIA data • The first step of peptide selection in antigen presentation by MHC class I molecules • Mascot tip – Peak list re-centroiding |
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February 2015 | • Quantify then identify or identify then quantify? • Deciphering Preferential Interactions within Supramolecular Protein Complexes: the Proteasome Case • Large Scale Proteomics symposium at Proteomic Forum 2015 • Mascot tip – Mascot Daemon data import filters |
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January 2015 | • The true benefit of high mass accuracy • Rapid Identification and Quantitative Validation of a Caffeine-Degrading Pathway in Pseudomonas sp. CES • Large Scale Proteomics symposium at Proteomic Forum 2015 |
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December 2014 | • Why can’t I search with more than 9 variable modifications? • Targeting Synaptic Pathology with a Novel Affinity Mass Spectrometry Approach • New patch for Mascot Server 2.5 |
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November 2014 | • How long should my search take? • Tracking Cancer Drugs in Living Cells by Thermal Profiling of the Proteome • Mascot Server 2.5 now available |
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