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Exercise PMF1


A PMF that gives a very strong match. Treat it as a standard and use it to find optimum settings for the search parameters


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A protein mixture (not alkylated) was separated on a 2D gel. A spot was extracted and digested with trypsin. The digest was analysed on a reflector MALDI-TOF instrument. The data file contains a list of MH+ values


Right-click this link: PMF1.txt, and choose Save target as or Save link as


  • The protein sequence is in SwissProt
  • A very strong match


  1. What is a suitable mass tolerance?
  2. What is the optimum setting for missed cleavages?
  3. Is there evidence for Met-Ox?


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