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Posted by Patrick Emery (September 30, 2025)

Top-3 Quantitation Summary in Mascot Daemon 3 using DDA-PASEF

Mascot Daemon includes a protein level quantitation summary report for both precursor based methods (LFQ, SILAC etc) and MS/MS quantitation (iTRAQ, TMT). The exported format is suitable for importing into statistic packages such as R or Perseus. In Mascot Daemon 3, we added an option to export protein intensity values calculated using the “Top-3″ method. Instead of using all matched [...]

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