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Articles tagged: hla
Processing Thunder-DDA-PASEF data with Mascot Distiller
Human leukocyte antigen (HLA) class I peptide ligands (HLAIps) are key targets in vaccine and immunotherapy development. They are also challenging to identify in standard mass-spec based proteomics; they are typically short, present at low abundance, and are not generated by a specific protease, requiring that search without enzyme specificity is conducted – dramatically increasing the search space compared with [...]
Identify more HLA peptides
Endogenous peptides are challenging to identify by database searching. A Mascot no-enzyme search matches every subsequence of a protein to the observed spectrum, which makes a very large search space even if precursor tolerance is tight. As a result, Mascot score thresholds tend to be conservative and sensitivity is reduced. Mascot ships with Percolator, which often improves discrimination between true [...]