Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

Blog

Articles tagged: MS2PIP

Return to blog archive

Posted by Ville Koskinen (November 25, 2024)

Best MS2PIP model for Thermo Orbitrap

Mascot Server 3.0 greatly improves protein and peptide identification rates with Thermo Orbitrap instruments. The new version ships with MS2PIP, which provides fragment intensity predictions. When the database search results are correlated with predicted spectra, it boosts the number of statistically significant matches even with straightforward tryptic digests. CID and HCD models For qualitative work and label-free quantitation, Mascot Server [...]

Full article and comments form

Posted by Ville Koskinen (October 14, 2024)

Tutorial: Selecting the best MS2PIP model

Mascot Server 3.0 can refine database search results using predicted fragment intensities. The predictions are provided by MS2PIP, and Mascot ships with suitable models for common instrument types. This tutorial shows how to select the best model for your instrument and experiment. What is MS2PIP? MS2PIP is a tool for predicting the MS/MS fragmentation spectrum from a peptide sequence, charge [...]

Full article and comments form

Posted by Ville Koskinen (August 14, 2024)

Predicted RT and fragment intensity in Mascot Server 3.0

A release candidate of the next version of Mascot Server is running on this website. One of the headline features in the preliminary release notes is refining results with machine learning, which includes integration with MS2Rescore. Below is a preview for you to enjoy while we are beta testing the new release. What is MS2Rescore? Mascot Server has shipped with [...]

Full article and comments form