Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

IIS configuration

For the Windows version of Mascot Server, we advise using IIS, Microsoft’s web server. It is essential to configure IIS correctly before installing Mascot Server. Ensure that all the options shown below are selected. Additional options can be selected if these are required for other applications.


Windows Server 2025

Launch Server Manager and select Add Roles and Features. In the Server Roles page of the wizard, check Web Server (IIS). In the Role Services page, configure as below. Ensure that, in addition to any other selections, all the checkboxes shown below are checked.

IIS configuration in Windows Server


Windows Server 2022

Procedure and options identical to Windows Server 2025.


Windows 11

In the Windows Start menu, find and run Turn Windows features on or off. Expand the node for Internet Information Services and ensure that, in addition to any other selections, all the checkboxes shown below are checked.

IIS configuration in Windows 11


Windows Server 2019

Procedure and options identical to Windows Server 2025.


Windows 10

Procedure and options identical to Windows 11.


Windows Server 2016

Procedure and options identical to Windows Server 2025.